Enzymatic Removal of Diacetyl from Beer

Cytosolic localization of acetohydroxyacid synthase Ilv2 and its impact on diacetyl formation during beer fermentation.

Diacetyl (2,3-butanedione) imparts an unpleasant "butterscotch-like" flavor to alcoholic beverages such as beer, and therefore its concentration needs to be reduced below the sensory threshold before packaging. We examined the mechanisms that lead to highly elevated diacetyl formation in petite mutants of Saccharomyces cerevisiae during beer fermentations. We present evidence that elevated diac...

Microbial and enzymatic methods of glucose removal from egg white Glucose removal from egg white

Enzymatic approach to phenol removal from wastewater using peroxidases

Introduction Phenol and its derivatives are present in various industrial effluents and possess a high level of toxicity even at minute concentrations. This could cause serious ecological problems if phenol-containing effluents are discharged untreated or partially treated into water bodies or land sites. Method Conventional wastewater phenol removal methods, which include biological treatment,...

Enzymatic removal and disinfection of bacterial biofilms.

Model biofilms of Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas fluorescens, and Pseudomonas aeruginosa were made on steel and polypropylene substrata. Plaque-resembling biofilms of Streptococcus mutans, Actinomyces viscosus, and Fusobacterium nucleatum were made on saliva-coated hydroxyapatite. The activity of enzymes against bacterial cells in biofilm was measured by fluoresc...

An automated distillation method for the determination of diacetyl in beer: a comparison of analysis by AutoAnalyzer and gas chromatography

was the case with the first design. Another advantage of the procedure described is that it enables the determination of total carbon and inorganic carbon in the range 10 to 50 mg/dm at a rate of 20 samples/h at a standard deviation of less than per cent. The described automated system is used very effectively in the authors laboratory for routine sample analysis. ACKNOWLEDGEMENTS This work wil...